Siberian Mouse Hd 132 20
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although the results of isoflavone feeding on siberian mice were unexpected, such responses in mice are more commonly found with experimental animals, notably rats and dogs, which lack the endogenous synthesis of the ligand estrogen.
the litkowitz-köch estimate of the age at which reproductive senescence occurs in the siberian male probably underestimates the start of reproductive senescence in male siberian mouse hd 132 20 several individuals by as much as three months. in such cases, the mating age is dropped by 3 months and so on for all subsequent mating attempts.. data used to estimate the age at which reproductive senescence occurs in the siberian male (which are also estimated for the entire population) are obtained by analysing the data of 173 mating attempts of 58 males and counting the number of mating attempts at each age. for this estimate, the age at which the initial reproduction ends in a male siberian mouse hd 132 20 is kept.
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Sections were dewaxed and rehydrated with graded ethanol series. Antigen retrieval was performed by incubation in citrate buffer (pH 6) using a microwave oven. Sections were permeabilised with Triton X (0.1% in PBS), blocked in buffer containing 10% non-immune rabbit serum and incubated with the primary antibody at 4 C overnight. The primary antibodies were: anti-beta-catenin (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA); anti-cadherin-1/N-cadherin (1:500, Abcam Plc, Cambridge, UK), and anti-vimentin (1:500, Abcam Plc, Cambridge, UK). Anti-alpha-catenin (1:50, supplied by Dr Michael Goldberg, Lund University, Sweden), goat anti-mouse IgG1 (1:500, BD Pharmingen, San Diego, CA), and anti-goat IgG (1:500, Jackson Immunoresearch, PA) were used as secondary antibodies.
For detection of germ cell nuclear antigen (GCNA), a marker of pre-diplotene oocytes of the first meiotic maturation in fetal and early postnatal mouse ovaries ( Enders & May 1994 ), paraffin sections were dewaxed, hydrated in PBS and a citrate antigen retrieval step was performed (0.01 M citrate buffer pH 6, 10 min microwave boiling). After cooling, sections were permeabilised with 0.1% Triton X and incubated for 15 min in 2% H2O2 in methanol to quench endogenous peroxidase. Following blocking for 1 h with 10% normal rabbit serum, sections were incubated with primary GCNA antibody (1:200, supplied by Dr George Enders, University of Kansas Medical Centre, Kansas City, KS, USA) for 1 h at room temperature. Sections were washed in PBS, then incubated with biotinylated secondary antibody (rabbit anti-rat 1:200 in 2% BSA in PBS) for 1 h. Binding sites were detected by incubation for 1 h with avidinbiotin-peroxidase, followed by exposure to diamino-benzidine (DAB) reaction for 10 s.