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GAP-43 messenger RNA expression during the early development of the goldfish spinal cord.
GAP-43, a membrane-associated guanine nucleotide-binding protein, plays a role in the regulation of neurite outgrowth. It was previously shown that the distribution of GAP-43 in the rat spinal cord correlates with the development of particular neuronal types. In this study, we examined the temporal and spatial expression of GAP-43 messenger RNA (mRNA) during early development in the goldfish spinal cord by in situ hybridization. Strong expression was detected during the earliest stages of neurulation (the neurula and the early neural rod stage), in the ventral region of the spinal cord, mainly in the dorsal column. During subsequent stages of neurulation and early neural tube closure, mRNA for GAP-43 was expressed mainly in the anterior ventral region of the spinal cord and the neural tube. By the segmentation stage (10 h post fertilization), GAP-43 expression was strongest in the ventral horn of the lumbar spinal cord. Strong expression was also detected in the medulla during the segmentation and hatching stages. This study is the first to show GAP-43 mRNA expression during the development of the goldfish spinal cord. The distribution of GAP-43 mRNA expression is similar to that of proteins in mammals, suggesting that GAP-43 might be a useful marker for the early development of the spinal cord and spinal ganglia in the goldfish.// Copyright 2019, The Go Authors. All rights reserved.
// Use of this source code is governed by a BSD-style
// license that can be found in the LICENSE.md file.
package cmp
import (
"fmt"
"reflect"
"github.com/google/go-cmp/cmp/internal/function"
)
// Option configures for comparison of transitively embedded struct values